|Neuroectoderm Differentiation||A chemically defined (Dual SMAD inhibition), feeder-free culture platform to uniformly differentiate hPSCs into early neurectoderm by 7 days.||2 weeks|
|Mesoendoderm/Cardiac Progenitor Differentiation||A chemically defined (GSK3 inhibitor and Wnt inhibitor), feeder-free culture platform to rapidly differentiate hPSCs into mesoendoderm by 1 day, and further to cardiac mesoderm by 7-9 days differentiation.||2 weeks|
|Definitive Endoderm Differentiation||A feeder-free differentiation platform to rapidly differentiate hPSC to definitive endoderm cells, which is the key step to drive the cells to various endoderm lineages, such as liver, pancreases, lung cells.||1 week|
a) qPCR data for various pluripotency and neuroectodermal markers at day 7 . b) Flow cytometry analysis of PAX6 expression in neuroectoderm cells at day 7.
a) Flow cytometry analysis of brachyury expression in mesoendoderm cells cultured with two different concentrations of CHIR at day 1. b) qPCR data for various pluripotency and mesoendoderm markers at day 1 with two different concentrations of CHIR. c) Immunofluorescence staining of Troponin T (red) and DAPI (blue) in cardiac mesoderm cells at day 9. d) Cardiac mesoderm cells beating at day 9.
Definitive Endoderm Differentiation
a) qPCR data for various pluripotency and definitive endoderm markers at day 3. b) Flow cytometry analysis of Sox17 expression in definitive endoderm cells at day 3. c) Immunofluorescence staining of FOXA2 (red), SOX17 (green), and DAPI (blue) in definitive endoderm cells at day 3.