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Training
Maintaining human pluripotent stem cells is a time consuming process that requires refined judgement and skill to maintain high-quality cultures. Genetic manipulation and/or differentiation of these cultures originate from complex procedures that may necessitate additional help to successfully obtain your wanted product. The SKI Stem Cell Research Facility offers various training programs for internal and external scientists looking to learn or better their skills in hPSC experiments
ServiceDescriptionTiming
hPSC Culture on Feeder Layer Hands-on training to perform the procedures involved in hPSC culture, specifically on MEF feeder layers. This includes preparing plates to contain MEF feeder layers, thawing, passaging and freezing hPSC cultures as well as selecting and manually picking hiPSC colonies from culture dish. We also offer training for characterization and quality control techniques specific to this service. One hour per day for one week
hPSC Culture on Feeder Free Condition Hands-on training to perform the procedures involved in hPSC culture, specifically on feeder free conditions. This includes coating plates with basement membrane matrix Matrigel, thawing, passaging and freezing hPSC cultures. We also offer training for characterization and quality control techniques specific to this service. One hour per day for one week
Differentiation of hPSC Hands-on training to perform differentiation(s) of hPSC cultures, which include neuroectoderm, mesoendoderm/cardiac progenitors, and definitive endoderm lineages. We also offer training for characterization and quality control techniques specific to this service. One hour per day for one week
Electroporation Training using the Lonza 4D Nucleofector to successfully learn and perform the process of electroporation, critical for gene editing of hPSC cultures. One to two hours